MIME-Version: 1.0 Content-Location: file:///C:/C484CAAD/GTTITTdataform.htm Content-Transfer-Encoding: quoted-printable Content-Type: text/html; charset="us-ascii" GTT

GTT/ITT<= o:p>

 

Date:

 

Time:

 

Age of Mice:

 

 

Mouse

Weight

Geno

Injection=

0 minutes=

15 minute= s

30 minute= s

60 minute= s

120 minut= es

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

* Glucose for GTT: 2grams/kg body weight (=3D1 unit/gram body weight)

* = Insulin for ITT: 0.8-1.2units/kg body weight (=3D1 unit diluted insulin/gram bo= dy weight)

Insulin dilutions from a 100 unit/mL stock

Final dose:

0.8 un= its/kg

1.0 un= its/kg

1.2 un= its/kg

1st dilut= ion

(final concentration: <= /p>

0.8-1.2 units/mL)

0.08mL stock into 10mL final volume of saline

0.1mL stock into 10mL final volume of saline

0.12mL stock into 10mL final volume of saline

Final dilution<= /o:p>

(0.1 units/mL)

1:10 dilution into saline

1:10 dilution into saline

1:10 dilution into saline