| region immediately following TAG stop codon of Irs1 | |||||||||||||||||||||||||||||||||||||||||||||
| ensembl-ucsc genomic | ------------------------------------------------------------------ | cgtaactggacatcacagcag | gtgcgtttttatggtggcaaagtcagaagacaaaagtgcttttaccatggggtgcttgagttctgttccttcccctccttctcttcccactccttcccacagtgtccttggggaggatactttttcaggaggtgtgggggtggggtggggaagggtcagggtacacagatgctcacctcgcattgacacctggcagatagtcaaagaactgtgtttggagcagccacagaagggcttctctct | ||||||||||||||||||||||||||||||||||||||||||
| ppNT3loxP (mock floxed) | ggtacccgccggccgctcgagataacttcgtataatgtatgctatacgaagttatggatccAATAG | cttaactggacgtcacagcag | gtgcgtttttatggtggcaaagtcagaagacaaaagtgcttttaccatggggtgcttgagttctgttccttcccctccttctcttcccactccttcccacagtgtccttggggaggatactttt-caggaggtgtgggggtggggtggggaagggtcagggtacacagatgctcacctcgcattgacacctggcagatagtcaaagaactgtgtttggagcagccacagaagggcttctctct | ||||||||||||||||||||||||||||||||||||||||||
| Kpn-Eag-Xho-loxP-Bam-(repeat of 5bp at stop: aatag) | 3' UTR (1) | gt splice donor | site of deletion | ||||||||||||||||||||||||||||||||||||||||||
| kis have above extra 66 bp | |||||||||||||||||||||||||||||||||||||||||||||
| but PCR products may differ by only 65 bp because of single bp deletion in pPNT3-Irs1-3loxP | |||||||||||||||||||||||||||||||||||||||||||||