Today's date:	9/19/05
PCR name:	Cre (E)
Purpose:	Determine presence of Cre transgene(s); does not distinguish between one copy or two
Samples:
1
2
3
4
5
			# of rxns
Reactions (ul):	X		4		Profile name:		Cre (E)
DNA	1				step	time (min:sec)	temp (C)
H20	13.5		54		1	15:00	95
10X buffer	2		8		2	:30	94
4mM dNTPs	1.5		6		3	:20	60
10pmol/ul primers					4	:45	72
567	0.5		2		go to	step 2	36 times
568	0.5		2		5	10:00	72
(control) 42	0.5		2		6	hold	4
(control) 43	0.5		2
	20	ul	76	ul
+ HotStar Taq	0.18	ul	0.72	ul
Primers:	Sequence: (5' to3')							Tm
567	ACCAGCCAGCTATCAACTCG							58.0
568	TTACATTGGTCCAGCCACC							55.8
(control) 42	CTAGGCCACAGAATTGAAAGATCT							56.3
(control) 43	GTAGGTGGAAATTCTAGCATCATCC							56.8
Products:	from allele(s)		length (bp)	digests?		primers
internal control	all		325			42 + 43
Cre Tg	Cre		200			567 + 568
Genotypes:	bands
+/+	325
Cre/+	325, 200
Cre/Cre	325, 200