Ptpn11 WT

Allele specific oligos for WT ('D') allele are in reverse orientation to give ~200 bp product

A

V

T

H

I

K

I

Q

N

T

G

D (61)

Y

Y

D

L

Y

G

G

E

K

F

33013

5'

GCT

GTT

ACC

CAC

ATC

AAG

ATT

CAG

AAC

ACT

GGG

GAC

TAC

TAT

GAC

CTC

TAT

GGT

GGG

GAG

AAG

GTG

3'

3'

CGA

CAA

TGG

GTG

TAG

TTC

TAA

GAC

TTG

TGA

CCC

CTG

ATG

ATA

CTG

GAG

ATA

CCA

CCC

CTC

TTC

CAC

5'

DR3

12/24 GC

3'

-TG

ATG

ATA

CTG

GAG

ATA

CCA

CCC

C

5'

33069

DR2

12/24 GC

3'

--C

CTG

ATG

ATA

CTG

GAG

ATA

CCA

CC

5'

33067

DR1

12/23 GC

3'

--A

CCC

CTG

ATG

ATA

CTG

GAG

ATA

C

5'

33063

Ptpn11 D61G

Allele specific oligos for 'G' allele are in forward orientation to give ~275 bp product

A

V

T

H

I

K

I

Q

N

T

G

G

Y

Y

D

L

Y

G

G

E

K

F

AgeI

33013

5'

GCT

GTT

ACC

CAC

ATC

AAG

ATT

CAG

AAC

ACC

GGT

GGC

TAC

TAT

GAC

CTC

TAT

GGT

GGG

GAG

AAG

GTG

3'

3'

CGA

CAA

TGG

GTG

TAG

TTC

TAA

GAC

TTG

TGG

CCA

CCG

ATG

ATA

CTG

GAG

ATA

CCA

CCC

CTC

TTC

CAC

5'

GF1

33024

5'

--C

ATC

AAG

ATT

CAG

AAC

ACC

GGT

GG

3'

11/24 GC

GF2

33022

5'

CAC

ATC

AAG

ATT

CAG

AAC

ACC

GGT

3'

11/24 GC

GF3

33019

5'

ACC

CAC

ATC

AAG

ATT

CAG

AAC

ACC

3'

11/24 GC

OUTER primers

276

DOF

32793

5'

GCC

AGC

CAT

TCA

AAG

TAG

AAT

CTC

3'

12/24 GC

203

GOR

12/24 GC

3'

AGT

CAC

TGT

AGA

AGC

ACT

GGT

CCA

5'

33222