Preparation of LB and LB+ Amp Plates

 

  1. use LB Agar Medium capsules
  2. add 6- capsules to 2L flask containing 1.5L of ddH2O
  3. autoclave for 45 minutes (liquid)
  4. wash and ethanol bench and set out sterile plates
  5. when flask is cool enough to touch with inner arm, add 1ml of Amp (common box 20C) per 1L of LB; swirl gently to mix
  6. pour coat plates
  7. dry 30 minutes to 1 hour
  8. mark plates as LB or LB+Amp (no mark for LB alone, black mark for LB+Amp)
  9. invert and store at 4Cin cold room