Preparation of LB and LB+ Amp Plates
- use LB
Agar Medium capsules
- add 6-
capsules to 2L flask containing 1.5L of ddH2O
- autoclave
for 45 minutes (liquid)
- wash
and ethanol bench and set out sterile plates
- when
flask is cool enough to touch with inner arm, add 1ml of Amp (common box –20C)
per 1L of LB; swirl gently to mix
- pour
coat plates
- dry 30
minutes to 1 hour
- mark
plates as LB or LB+Amp (no mark for LB alone, black mark for LB+Amp)
- invert
and store at 4Cin cold room