MAKING 100 mM HISTIDINOL FOR
SELECTION OF MAMMALIAN CELL CULTURES
A. Make 100 mM Hepes in T.C. grade water, pH to 7.4 with NaOH.
B. Add L-Histidinol to Hepes (Sigma #H 6647) to 100 mM.
C. Readjust pH to 7.4 with NaOH.
D. Filter sterilize using 0.2 m vacuum unit.
E. Refrigerate the solution if it is to be used soon, or freeze aliquots for long-term (>2 week) storage.
F. Final concentration for selection 7-10 mM; for carrying cells post selection 5 mM.