Morris’s MICE FROZEN @ Jax as of August 2012,

Projects 22844 (09/2011),  24467 (05/2012) and 25154 (07/2012).

Strain Name

Stock #

Qty Straws

% to 2-cell

Date of Sperm freeze

Storage  Contract End Date

NOTE

Irs1G965R

908884

19

87.7

09/27/11

September 2013

1

LrbCre:fIrs2

908885

19

88.0

09/27/11

September 2013

2

MipGFP:fIrs2   (pureC57)

908886

19

71.9

09/27/11

September 2013

3

RipCre:fIrs1:fIrs2:RosaGFP

908887

19

43.6

09/27/11

September 2013

4

CaIICre:NeoIrs2   (NO CreER)

908888

19

72.7

09/27/11

September 2013

5

Ngn3Cre:fFoxo1

908889

19

76.8

09/27/11

September 2013

6

RipCreER: fFoxo1:NeoIrs2:LacZ

908890

19

80.3

09/27/11

September 2013

7

mTCreER:fFoxo1:Irs2KO:LacZ

908891

19

71.1

09/27/11

September 2013

8

Irs4KO

908892

19

66.7

09/28/11

September 2013

9

Irs2KO

908893

19

79.8

09/28/11

September 2013

10

LiverCre:fIrs1:fIrs2 fFoxo1

910291

19

30.1

05/17/12

June 2014

11

LiverCre:fIrs1:fIrs2

910290

19

61.0

05/18/12

June 2014

12

CaIICreER

910288

19

54.7

05/17/12

June 2014

13

Irs1FCTxCK

910287

19

73.0

05/18/12

June 2014

14

Irs1JBMxCK

910286

19

51.8

05/18/12

June 2014

15

Irs1S307AxCK

910285

19

61.9

05/18/12

June 2014

16

Irs1S302AxCK:Tscl lox/lox

910284

19

45.0

05/18/12

June 2014

17

PtenKO

910283

19

65.1

05/17/12

June 2014

18

Irs1KO

910282

19

68.7

05/17/12

June 2014

19

Irs3KO

910281

19

66.0

05/17/12

June 2014

20

B6-Tg-NSE-Irs2

910280

19

65.0

05/18/12

June 2014

21

fFoxo1-3-4

910279

19

80.3

05/18/12

June 2014

22

Irs2Tg15   (RETINA)

910278

19

51.0

05/17/12

June 2014

23

LIRKO

910277

19

62.3

05/24/12

June 2014

24

fPGC-1b:AlbCre

910276

19

72.5

05/24/12

June 2014

25

MckCre:Irs1(f/+):fIrs2:fFoxO1:fFoxO3

910293

18

50.0

05/17/12

July 2014

26

MckCre:fIrs1(f/+):fIrs2

910292

18

75.7

05/17/12

July 2014

27

NestinCre:fIrs2

910289

19

5.8

05/18/12

June 2014

28

ASCre (Project # 25154)

910660

19

6/27/12

91.0

August 2014

29

ASCre:floxSF1 (Project # 25154)

910661

19

6/27/12

72.0

August 2014

30

floxBetacatenin (Project # 25154)

910662

19

6/27/12

22.9

August 2014

31

mTertrtTA:tetCre (Project # 25154)

910663

19

6/27/12

43.0

July 2014

32

mTertCreER:fRosaLacZ (Project # 25154)

910664

19

6/27/12

60.2

August 2014

33

mTertGFP22 (Project # 25154)

910665

19

6/27/12

72.6

July 2014

34

MckCre:fFoxo1:fFoxo3 (Project # 25154)

910666

19

6/25/12

81.7

July 2014

35

AlbCre:fFoxo1 (Project # 25154)

910667

19

6/25/12

39.4

August 2014

36

SirtTg (Project # 25154)

910668

19

6/25/12

80.8

July 2014

37

fIrs1:fIrs2:SirtTg (Project # 25154)

910669

19

6/25/12

58.1

July 2014

38

fIrs1:fIrs2:fFoxo1:fFoxo3 (Project # 25154)

910671

19

6/25/12

54.5

August 2014

39

AlbCre:fFoxo1:Ob/+ (Project # 25154)

910672

19

6/25/12

61.7

July 2014

40

 

Recovery Success Levels

Excellent (Above 66%) - Strain can be recovered in abundant quantities with extra flexibility to quickly build a colony.

Good (41-65%) - Strain can be recovered in abundant quantities to build a colony.

Fair (20-40%) - Strain can be recovered in sufficient quantities to build a colony.

QCL recommended (10-20%) – Strain may be at risk. It should be recovered to liveborn to determine the quality of the frozen sperm and evaluate what work will be required and what costs above standard, incurred to recover and build a colony.

Freeze Backup (below 10%) - Strains producing less than 10% 2-cells may be challenging to recover by standard IVF methods. Consider repeating the cryopreservation with new males